Johannes Schnur
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Characterizing the Leakage Mechanism of Thermoresponsive Liposomes using Time-Resolved FluorescencePrincipal investigator: Prof. Dr. Heiko Heerklotz Institute of Pharmaceutical Sciences Phone: +49 (0) 761-203-6336 |
Abstract
The major drawbacks in conventional tumor therapy are its high systemic toxicity and the low bioavailability of the drug molecules at the tumor site. Thermoresponsive Liposomes (TSLs) are capable of delivering the drug to the tumor site and release their content when triggered by mild hyperthermia (40—42°C).
While TSLs have been investigated since the 1970s, only little is known about their physicochemical properties, and how their components interact with blood proteins. We used Time-Resolved Fluorescence, Dynamic Light Scattering, Differential Scanning Calorimetry, and Isothermal Titration Calorimetry to systematically show the influence of various common constituents of TSLs on the phase- and release-behavior of TSLs. This will allow a more rational approach to design TSL formulations for new APIs.
Methods
Time-Resolved Fluorescence, Dynamic Light Scattering, Differential Scanning Calorimetry, Isothermal Titration Calorimetry
