Andrea Origi
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An alternative, SRP- and SecA independent pathway,
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Abstract
The correct localization of proteins to their cellular destination is essential for sustaining cell viability in eukaryotic and prokaryotic organisms. Approximately 30% of all proteins synthesized in the cytosol engage the protein transport machineries in the endoplasmic reticulum (ER) membrane in eukaryotic cells or those in the cytoplasmic membrane in bacteria. While the recognition of proteinaceous signals during protein transport is well established, the contribution of signals within the mRNA and their targeting is largely unknown.
Methods
- Membrane protein expression and purification
- mRNA Localization with SMI (single molecule imaging)
- MS2-GFP method
- mRNA Synthesis and purification
- Site directed and chemical cross-linking
- In-Vitro protein synthesis
Publications
Denks, K, Sliwinski, N, Erichsen, V, Borodkina, B, Origi, A and Koch, HG (2017) The signal recognition particle contacts uL23 and scans substrate translation inside the ribosomal tunnel. Nature Microbiology, 2, 16265.
