Stefan Dannenmaier
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Study of the universally conserved Obg1-like ATPase 1
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Abstract
Obg-like ATPase 1 (Ola1) is a highly conserved GTPase that belongs to the Obg-related class of P-loop GTPases. The subfamily of Ola1 deviates from other GTPases as it preferentially hydrolyzes ATP over GTP. Proteins of the Ola1 family are involved in various cellular processes ranging from transcriptional and translational regulation, to cellular stress responses and promotion of tumor growth and metastasis. However, the exact role of Ola1 in the aforementioned processes remains elusive and requires further investigation.
In my project, we analyze the role of Ola1p from Saccharomyces cerevisiae in protein homeostasis, in particular its impact on the cellular response towards severe heat stress using a combination of classical biochemical methods with fluorescence confocal microscopy and quantitative high-resolution mass spectrometry.
Methods
- Molecular biology and yeast genetics
- Affinity purification
- Mass spectrometry
- Fluorescence microscopy
