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Andrea Origi

Andrea Origi

An alternative, SRP- and SecA independent pathway,
for membrane protein targeting

Principal Investigator: Prof. Dr. Hans-Georg Koch

Institute for Biochemistry and Molecular Biology
Stefan-Meier-Straße 17
79104 Freiburg

Phone: +49 (0) 761 - 203



The correct localization of proteins to their cellular destination is essential for sustaining cell viability in eukaryotic and prokaryotic organisms. Approximately 30% of all proteins synthesized in the cytosol engage the protein transport machineries in the endoplasmic reticulum (ER) membrane in eukaryotic cells or those in the cytoplasmic membrane in bacteria. While the recognition of proteinaceous signals during protein transport is well established, the contribution of signals within the mRNA and their targeting is largely unknown.


  • Membrane protein expression and purification
  • mRNA Localization with SMI (single molecule imaging)
  • MS2-GFP method
  • mRNA Synthesis and purification
  • Site directed and chemical cross-linking
  • In-Vitro protein synthesis


Denks, K, Sliwinski, N, Erichsen, V, Borodkina, B, Origi, A and Koch, HG (2017) The signal recognition particle contacts uL23 and scans substrate translation inside the ribosomal tunnel. Nature Microbiology, 2, 16265.