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Selected Methods in Biophysical Chemistry

The course addresses mainly fluorescence spectroscopy (primarily TCSPC), microcalorimetry (ITC, DSC, PPC), dynamic light scattering and zeta potential measurements (NIBS, PALS), Langmuir monolayer techniques and asymmetric field-flow-field fractionation (AF4). Each method is introduced with respect to its background and examples are explained for its use in biophysical chemistry, mostly related to lipid membrane systems. Then, a demonstration and, where possible, some hands-on introduction is done on state-of-the-art instrumentation. The class is small and very interactive – potential, specific aims and problems of participants can be discussed with respect to the applicability of the methods presented.  

Teaching goals:


Relatively deep insight into the background, possible applications (and limitations) of a few biophysical methods.
Instructor: H. Heerklotz, M. Hoernke, M. Holzer
Duration: 1 semester, 2h/week (run as 4h biweekly)
Participants: offered to all students of the Uni FR M.Sc. programmes in Biophysics and Biochemistry, Pharmaceutical Sciences, Chemistry, and doctoral researchers of the RTG 2202.
Location: Pharmacy building, H.-Herder-Str. 9, labs and room t.b.a.


The course is offered in the summer term, if you want to participate, contact your coordinator.