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Shuo Liang

Shuo Liang

 

Studies of the structure and function of sodium/proton-exchangers
and their interaction with regulatory proteins

Principal investigator: Prof. Dr. Carola Hunte

Institut für Biochemie und Molekularbiologie
Stefan-Meier-Str. 17
79104 Freiburg

Phone: +49 (0) 761-203-97495
shuo.liang@biochemie.uni-freiburg.de

 

Abstract

Homeostasis of the intracellular pH is critical for cell vitality. A key player in this process is the Na+/H+ exchanger NHE1, which exports excess protons in exchange to sodium ions. The interaction of NHE1 with calcineurin B homologous proteins (CHPs) is important for full functionality of this transporter. Inherited loss-of-function mutations that are linked to diseases are known for NHE1 as well as for a CHP isoform. Three human CHP isoforms exist. They bind to the cytoplasmic regulatory domain of NHE1. In order to gain insights in the so far unknown energetics of these interactions, we produced and purified human CHPs as well as characterized their interaction of NHE1 with biochemical and biophysical methods. The data provide insights into the molecular mechanisms that underlie the regulatory interaction between CHPs and NHE1.

 

Methods

  • Recombinant proteins expression and purification
  • Protein stability analysis
  • Fluorescence spectroscopy
  • Protein-protein interactions